In the pilot episode of The 13 Ghosts of Scooby Doo, the gang crash lands at the Himalayas, where they walk into a tavern inhabited by people with a dark secret: they are cursed to turn into werewolves under a full moon. Daphne takes a sip of what is revealed to be freshly squeezed wolfsbane, which Scrappy states turns people into werewolves, but Daphne rebuffs the claim. After the townspeople become werewolves, Daphne is shown in front of the full moon beginning her transformation. She grew hair and claws on her hands, her face became animalistic, her fangs elongated, and her muzzle extended to complete her villainous werewolf transformation. Daphne later led the other werewolves against her former allies, establishing Daphne's heel turn. After her transformation, the evil Daphne snarled at Shaggy and Scooby and went after the pair, as well as Scrappy and Flim Flam, after escaping through a pothole. Flim Flam drenches Daphne with his "Lotsa Luck Joy Juice," which turns her back into her human form.